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Extra info for Abscess - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References
Vaccines or chemoprophylactic agents, which can protect residents of endemic areas or travelers, are not available. Infection is acquired by ingestion of the cyst form, followed by excystation of amoeboid trophozoites, which migrate to and colonize the bowel lumen. The endosomal and lysosomal (endo-lysosomal (EL)) system of Entamoeba appears to play a role in its pathogenesis as (I) uptake and digestion of nutrients, (ii) invasion of the intestinal epithelium, and (iii) dissemination and establishment of extra-intestinal infections, including liver abscess, rely on endocytosis and the action of hydrolytic enzymes and pore-forming proteins secreted from the pathogen.
Source: European Radiology. 2003 July; 13(7): 1750-2. Epub 2003 February 07. cmd=Retrieve&db=pubmed&dopt=A bstract&list_uids=12835993 44 Abscess • Brodie's abscess of the cuboid bone: a case report. Author(s): Bagatur AE, Zorer G. Source: Clinical Orthopaedics and Related Research. 2003 March; (408): 292-4. cmd=Retrieve&db=pubmed&dopt=A bstract&list_uids=12616073 • Brucella pelvic tubo-ovarian abscess mimicking a pelvic malignancy. Author(s): Seoud MA, Kanj SS, Habli M, Araj GF, Khalil AM. Source: Scandinavian Journal of Infectious Diseases.
In Specific Aim 2 the role of the EhRabs in EL function and pathogenicity will be addressed. Genetically engineered Entamoeba cell lines overexpressing dominant inhibitory and constitutively active versions of the EhRabs will be generated. In addition, Entamoeba cell lines expressing anisense transcripts of the EhRabs (to reduce the cellular levels of the EhRab) will be generated. EL processes will be examined in these strains, including pinocytosis of fluid phase and phagocytosis of large particles, maintenance of intra-endosomal pH, and secretion of hydrolases.