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Antennas and Reaction Centers of Photosynthetic Bacteria: by H. Zuber (auth.), Professor Dr. Maria Elisabeth

By H. Zuber (auth.), Professor Dr. Maria Elisabeth Michel-Beyerle (eds.)

The workshop on "Antennas and response facilities of Photosynthetic Bac­ teria" used to be held at Feldafing, Bavaria (F. R. G. )' March 23-25, 1985. This workshop focussed on fundamental procedures with emphasis on constitution, inter­ activities and dynamics. It assessed structural, spectroscopic and dynamic information that have gathered lately, supplying an summary of the mech­ anism of the purchase, garage and priceless disposal of power in bacterial photosynthesis. This quantity is a checklist of the invited papers provided on the workshop. the fabric was once equipped into 5 sections: I. Antennas: constitution and effort move II. response facilities: constitution and Interactions III. Electron move: conception and version structures IV. response Cen,ters: constitution and Dynamics V. version platforms on functionality of Antennas and response facilities i want to precise my gratitude to all of the members within the paintings­ store for his or her contributions, and to the authors for the well timed education in their manuscripts. i'm indebted to the contributors of the organizing committee, Professors Sighart F. Fischer and Hugo Scheer for his or her most dear tips and recommendation. The workshop shouldn't have been such a success with no assistance from my secretary, Frau Petra KahlfuB, and my coworkers in its association. I thank Frau KahlfuB fairly additionally for her tips within the practise of those lawsuits. The workshop was once geared up below the auspices of the Technical Uni­ versity of Munich, the Max-Planck-Society and the collage of Munich.

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They had an absorption maximum at 635 nm with a strong shoulder around 620 nm and an emission maximum at 670 nm. Phycoerythrocyanin was isolated from green-lightgrown cells following the protocol described for the isolation of phycocyanin [12]. It elutes from OEAE-cellulose column as the first band. The aggregation state was determined by ultracentrifugation from the sedimentation coefficients. The data were analyzed as described earlier [12]. 4. Results and Discussion The isotropic fluorescence decay curves displayed in figure la are recorded with 600 nm excitation but different spectral observation windows (cut-off filters).

This fast 29 B 400 aDo IZOO 1600 TIME (pel Figure 1: Dependence of fluorescence decay of functionally intact phycobilisomes isolated from Mastigocladus laminosus on observation window. Cut-off filters used are: 620 nm (A), 630 nm (B), 645 nm (C) and 665 nm (D). Excitation wavelength in all cases: 600 nm. The parameters of the fit curves are listed in table 1. transfer route is absent in the monomeric unit and should, according to energetic criteria, be slowed down in phycoerythrocyanin (PEC). In the latter chromoprotein, the oc-phycocyanobilin is replaced by a phycoerythrocyanobilin, which absorbs around 568 nm.

4 6 M [ureal 8 " .......... 7 8 M [ureal Fig. •. ) from M. laminosus with urea. Relative absorptions at the long- wavelength (top) and near-UV maxima (bottom), normalized to the absorptions in the absence of urea. All spectra were recorded from stock samples treated separately before the measurement with the appropriate amount of urea, either as 8M solution or as solid. 5, T= 25°C. Fig. 7: Denaturation of PC from M. laminosus and its subunits with urea. Relative fluorescence intensities corrected for absorption.

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