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Atlas of Human Anatomy In Three Volumes by R. D. Sinelnikov, Ludmila Aksenova

By R. D. Sinelnikov, Ludmila Aksenova

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1d). A second development was the design of three-dimensional (3D) DNA origami structures. The first approach demonstrated that DNA origami sheets can be arranged in 3D by geometry-inducing crossovers to form a DNA origami box with a controllable lid (Fig. 1e) [8]. The second approach was to stack DNA origami sheets to form solid 3D shapes (Fig. 1f) [9], which could be further curved and twisted into more complicated shapes (Fig. 1g) [10]. The curvature and bending of single-layer DNA origamis could also be controlled by choosing crossover positions to enforce an overall shape (Fig.

Characterized in vitro, in vivo, and in silico [5]. We formulated a model-driven process to engineer static, ribozyme-regulated expression devices (rREDs) and dynamic, metabolite-responsive, aptazyme-regulated expression devices (aREDs) with quantitatively predictable functions in E. coli. rREDs and aREDs have immediate usefulness as biosensors and controllers for engineered metabolic pathways and could serve as the bases for constructing very large and complex synthetic biological systems. Crucially, the physical implementation of functional devices in that work required the development of a novel method for designing transcripts with kinetic RNA folding simulations.

Nat Protoc 6:1669–1682 24. Pettersen EF, Goddard TD, Huang CC et al (2004) UCSF Chimera—a visualization system for exploratory research and analysis. J Comput Chem 25:1605–1612 25. Doppelt-Azeroual O, Moriaud F, Adcock SA, Delfaud F (2009) A review of MED-SuMo applications. Infect Disord Drug Targets 9:344–357 26. Jambon M, Andrieu O, Combet C et al (2005) The SuMo server: 3D search for protein functional sites. Bioinformatics 21:3929–3930 27. Maggiora GM, Shanmugasundaram V (2011) Molecular similarity measures.

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